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Journal Articles

Comparative radiation tolerance based on the induction of DNA double-strand breaks in Tobacco BY-2 cells and CHO-K1 cells irradiated with $$gamma$$ rays

Yokota, Yuichiro; Shikazono, Naoya; Tanaka, Atsushi; Hase, Yoshihiro; Funayama, Tomoo; Wada, Seiichi; Inoue, Masayoshi*

Radiation Research, 163(5), p.520 - 525, 2005/05

https://doi.org/10.1667/RR3355
 Times Cited Count：20 Percentile：50.54(Biology)

Higher plants are generally more tolerant to ionizing radiation than mammals. To explore the radiation tolerance of higher plants, the amount of DNA double-strand breaks (DSBs) induced by $$gamma$$-rays was investigated in tobacco BY-2 cells and compared with that investigated in Chinese hamster ovary (CHO)-K1 cells as a reference. The resulting DNA fragments were separated by pulsed-field gel electrophoresis and stained with SYBR Green I. Initial DSB yield was then quantified from the fraction of DNA fragments shorter than 1.6 Mbp based on the assumption of random distribution of DSBs. The DSB yield in tobacco BY-2 cells (2.0 $$pm$$ 0.1 DSBs Gbp$$^{-1}$$ Gy$$^{-1}$$) was only one-third of that in CHO-K1 cells. Furthermore, the calculated number of DSBs per diploid cell irradiated with $$gamma$$-rays of mean lethal dose was five times greater in tobacco BY-2 cells (263.2 $$pm$$ 13.2) than in CHO-K1 cells. These results suggest that the radiation tolerance of tobacco BY-2 cells appears to be due to not only a lower induction of DNA damage but also a more efficient repair of the induced DNA damage.

Journal Articles

Light dependency of resistance to ionizing radiation in ${it Euglena gracilis}$

Hayashi, Hirotaka; Narumi, Issei; Wada, Seiichi; Kikuchi, Masahiro; Furuta, Masakazu*; Uehara, Kaku*; Watanabe, Hiroshi*

Journal of Plant Physiology, 161(10), p.1101 - 1106, 2004/10

https://doi.org/10.1016/j.jplph.2004.04.005
 Times Cited Count：10 Percentile：23.1(Plant Sciences)

The resistance of ${it Euglena gracilis}$ strains Z (wild type) and SM-ZK (chloroplast-deficient mutant) to ionizing radiation was investigated. The colony forming ability of ${it E. gracilis}$ strain Z was higher than that of strain SM-ZK after $$gamma$$-irradiation. For both strains, the resistance of light-grown cells was higher than that of dark-grown cells, suggesting that the light conditions during the culture contribute to the radiation resistance of ${it E. gracilis}$. The comet assay showed that the ability of rejoining DNA double-strand breaks (dsb) was much higher in the light-grown cells. These results suggest that ${it E. gracilis}$ possesses a light-induced repair system to cope with DNA dsb.

Oral presentation

Secondary structural changes of histone proteins in X-irradiated cells

Yamamoto, Satoshi; Izumi, Yudai; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Secondary structural changes of chemically modified histones in human cells exposed to X-rays

Yamamoto, Satoshi; Izumi, Yudai; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

no abstracts in English

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